Label-free cell sorting based on intrinsic cell properties

Selective enrichment of cell populations based on intrinsic mechanical and morphological properties — without labels, antibodies, or prior sample preparation.

Developed by ZELLMECHANIK DRESDEN for academic research.

Why label-free sorting?

  • Preserves native cell state

  • No reagents, no bias

  • Compatible with downstream assays

Especially relevant for fragile, primary, or heterogeneous cell populations.

How it works

Experimental demonstration of size-based microbead sorting in a microfluidic channel, captured with an extended field of view to visualize the sorting event.

  1. Measure intrinsic properties (size, deformation, morphology)

  2. Decide in real time

  3. Sort mechanically, label-free

Based on real-time deformability cytometry (RT-DC).

Representative results

Scatter plot of cell deformation versus cell area showing label-free cell sorting. Before sorting, two overlapping populations are visible; after size-based sorting, a narrow, enriched population remains.

Enriched lymphocytes from a leucocyte sample

Representative data showing label-free size-based enrichment of a cell population. The target fraction increased from 2174 / 4218 cells (51.6%) before sorting to 558 / 564 cells (98.9%) after sorting. Reduced event count reflects selective gating.

Talk to us about your applications.

About this technology

It is

  • Label-free enrichment

  • Property-based sorting

  • Research-focused technology

It is not

  • A replacement for high-throughput FACS

  • Antibody-based phenotyping

  • Clinical-grade sorting

Product link

Label-free sorting is available as a module integrated into ZELLMECHANIK DRESDEN systems.

View the SortingModule
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